Product name:Human CCL4 ELISA Kit
Description:
This assay employs an antibody specific for human CCL4 coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP-beta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CCL4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CCL4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.