Product name:Rat Neuroglobin,NGB ELISA Kit
Application:ELISA
Quantity:96T
Species:Rat
Restrictions:For Research Use only.
Descripton:
Intended use:This immunoassay kit allows for the specific measurement of rat NGB concentrations in cell culture supernates, serum, and plasma.
Introduction:Neuroglobin (NGB) is a newly discovered member of the hemoglobin superfamily that is primarily expressed in the brain of humans and other vertebrates. It is an O2-binding protein localized to cerebral neurons of vertebrates, including humans. Its physiological role is unknown but, like hemoglobin, myoglobin, and cytoglobin/histoglobin, it may transport O2, detoxify reactive oxygen species, or serve as a hypoxia sensor.
Test principle:This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for NGB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NGB present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for NGB is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NGB bound in the initial step. The color development is stopped and the intensity of the color is measured.