Product name:Rat tartrate-resistant acid phosphatase 5b,TRACP-5b ELISA Kit
Description:
Intended use
This immunoassay kit allows for the specific measurement of rat tartrate-resistant acid phosphatase orm 5b (TRACP-5b) concentrations in serum and plasma.
Test principle
This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TRACP-5b has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TRACP-5b present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for TRACP-5b is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRACP-5b bound in the initial step. The color development is stopped and the intensity of the color is measured.