Product name:Rat Thrombin activatable fibrinolysis inhibitor,TAFI ELISA Kit
Application:ELISA
Quantity:96T
Species:Rat
Detection range:12.5-800 ng/mL
Sensitivity:3.2 ng/mL
Restrictions:For Research Use only.
Descripton:
Intended use:This immunoassay kit allows for the in vitro quantitative determination of rat TAFI concentrations in serum, plasma and other biological fluids.
Introduction:TAFI, Thrombin Activatable Fibrinolytic Inhibitor, (also known as carboxypeptidase U and plasma carboxypeptidase B) is a 60,000 D molecular ratio glycoprotein (proenzyme form) present in rabbit plasma1 that modulates fibrinolysis in vivo. This proenzyme is converted to a 35,000 D molecular ratio active form, TAFIa, following proteolytic cleavage by the thrombin/ thrombomodulin complex.
Test principle:The microtiter plate provided in this kit has been pre-coated with an antibody specific to TAFI. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of TAFI in the samples is then determined by comparing the O.D. of the samples to the standard curve.